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MARCH6 antibody (Membrane-Associated Ring Finger (C3HC4) 6, E3 Ubiquitin Protein Ligase) (Internal Region)

Details for Product anti-MARCH6 Antibody No. ABIN238654, Supplier: Log in to see
Antigen
  • RGD1310927
  • RGD1565757
  • im:7161807
  • wu:fa98a02
  • si:dkey-40c17.2
  • Mar-06
  • 3830408G03
  • F830029L24Rik
  • mKIAA0597
  • DOA10
  • MARCH-VI
  • RNF176
  • TEB4
Epitope
Internal Region
12
12
7
7
4
3
1
1
Reactivity
Human
50
20
14
1
1
1
1
1
1
1
Host
Goat
31
14
6
Clonality
Polyclonal
Conjugate
This MARCH6 antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
33
24
11
6
2
1
Supplier
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Immunogen Peptide with sequence C-QGRRVIFQKVKE, from the internal region of the protein sequence according to NP_005876.2.
Blocking Peptide Blocking peptide for this product available: ABIN369640
Cross-Reactivity Mouse (Murine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name TEB4 / MARCH-VI (MARCH6 Antibody Abstract)
Background Synonyms: MARCH-VI, TEB4, MARCH6, membrane-associated ring finger (C3HC4) 6, KIAA0597, RNF176, membrane-associated RING-CH protein VI
Gene ID 10299, 223455
NCBI Accession NP_005876
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 100 kDa band observed in Human Lung lysates (calculated MW of 103 kDa according to NP_005876.2). Recommended concentration: 0.2-0.5 µg/mL. An additional band of unknown identity was also consistently observed at 18 kDa. This band was successfully blocked by incubation with the immunising peptide.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-MARCH6 antibody (Membrane-Associated Ring Finger (C3HC4) 6, E3 Ubiquitin Protein Ligase) (Internal Region) (ABIN238654) ABIN238654 (0.2 ug/ml) staining of Human Lung lysate (35 ug protein in RIPA buffer). ...
Background publications Kreft, Wang, Hochstrasser: "Membrane topology of the yeast endoplasmic reticulum-localized ubiquitin ligase Doa10 and comparison with its human ortholog TEB4 (MARCH-VI)." in: The Journal of biological chemistry, Vol. 281, Issue 8, pp. 4646-53, 2006 (PubMed).

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