Mannose-6-Phosphate Receptor (Cation Dependent) (M6PR) antibody

Antigen: Mannose-6-Phosphate Receptor (Cation Dependent) (M6PR)

Synonyms: SMPR, MPR46, CD-MPR, FLJ32994, CD222, CI-MPR, Mpr300, AI661837, M6P/IGF2R, M6PR, CDMPR, MGC140730, MGC94300, m6pr, MGC68896, MGC130765, zgc:77757, wu:fj81h11, MGC89423, mprd

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN238667

Additional Information

Alternative name: M6PR

Gene ID: 4074, 17113 (mouse), 312689 (rat)

Immunogen: KGGDEYDNHCGKEQ

Format: Affinity-purified

Description: All names and symbols: M6PR, mannose-6-phosphate receptor (cation dependent) , CD-MPR, FLJ32994, MPR46, SMPR, 46-kDa mannose 6-phosphate receptor, Mr 46,000 Man6PR, cation-dependent mannose-6-phosphate receptor. Official Symbol: M6PR. RefSeq number(s): NP_002346.1

Specificity: Human, Mouse, Rat, Dog, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Preliminary experiments gave bands at approx 70kDa and 35kDa in Human Lymph Node and Thyroid Gland lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 31.0kDa according to NP_002346.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Zygmunt, McKinnon, Herr et al.: "HCG increases trophoblast migration in vitro via the insulin-like growth factor-II/mannose-6 phosphate receptor." in: Molecular human reproduction, Vol. 11, Issue 4, pp. 261-7, 2005 (PubMed).