NCEH1 antibody (C-Term)

Catalog No. ABIN238671

Product Details anti-NCEH1 Antibody

Target: NCEH1 (Neutral Cholesterol Ester Hydrolase 1 (NCEH1))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NCEH1 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: AADACL1

Sequence: RTRNSYIKWL DQN

Cross-Reactivity: Cow, Dog, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-RTRNSYIKWLDQN, from the C Terminus of the protein sequence according to NP_065843.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments gave an approx 26-28 kDa band in A431 cell lysates and in Human Hippocampus lysates after 1 μg/mL antibody staining. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 h

Peptide ELISA: antibody detection limit dilution 1:1000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the Endoplasmic reticulum of A431 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended con

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NCEH1

Target: NCEH1 (Neutral Cholesterol Ester Hydrolase 1 (NCEH1))

Alternative Name: AADACL1 (NCEH1 Products)

Synonyms: AADACL1 antibody, NCEH antibody, Aadacl1 antibody, B230106I24Rik antibody, CPO-BP antibody, Nceh antibody, RGD1311104 antibody, nceh antibody, aadacl1 antibody, MGC79479 antibody, nceh1b antibody, zgc:153038 antibody, zgc:92416 antibody, neutral cholesterol ester hydrolase 1 antibody, neutral cholesterol ester hydrolase 1 S homeolog antibody, neutral cholesterol ester hydrolase 1b, tandem duplicate 1 antibody, neutral cholesterol ester hydrolase 1a antibody, NCEH1 antibody, Nceh1 antibody, nceh1 antibody, nceh1.S antibody, nceh1b.1 antibody, nceh1a antibody

Background: AADACL1, arylacetamide deacetylase-like 1

Gene ID: 57552, 320024

NCBI Accession: NP_065843, NP_001139748, NP_001139749