CCK-A Receptor (CCKAR) antibody

Antigen: CCK-A Receptor (CCKAR)

Synonyms: CCK-A, CCKRA, CCK1-R

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN239055

Additional Information

Alternative name: CCK-A Receptor

Immunogen: Synthetic peptide of rat CCK A R (a.a. 256 - 267)

Description: The physiologic actions of the cholecystokinin (CCK) family of peptide hormones are mediated through two classes of G protein-coupled receptors GPCR), CCK-A R and CCK-B R. CCK-A R is a 428 amino acid protein with 7 putative transmembrane domains. This GPCR is involved in gall bladder contraction, insulin secretion, and has also been described in specific regions of the central nervous system. CCK-A R has been implicated in pancreatic tumorigenesis as well as the pathogenesis of eating disorders and drug addiction.

Specificity: In immunohistochemistry experiments this antibody detects CCK-A R in rat, mouse and human tissues. Reactivity with CCK-A R from other species has not been determined.

Application Details

Application Notes: Immunohistochemistry: This antibody can be used on human formalin-fixed paraffin-embedded tissue sections and on mouse and rat cryostat tissue sections. The recommended working dilution is 1.5 - 15 ug/mL. Labeling for CCK-A R can be seen in the cytoplasm and plasma membrane of positive cells. Heat-induced epitope retrieval techniques may be required to improve intensity of CCK-A R labeling. Western blotting: This antibody can be used to immunoblot lysates from rat, mouse and human tissues at a recommended working dilution of 0.1 ug/mL. Positive control tissues: Pancreas, muscularis propria of antrum, fundus, gallbladder, and kidney.

Concentration: 1mg/ml

Storage: Antibody can be aliquotted and stored frozen at -20° C to -70° C in a manual defrost freezer for six months without detectable loss of activity. The antibody can be stored at 2° - 8° C for 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Research Area: Receptors

Restrictions: For Research Use only