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Antibodies
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anti-Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) antibody
Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) antibody
| Antigen |
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| Synonyms |
MACS, 80K-L, PKCSL, PRKCSL, FLJ14368, FLJ90045, KINC, Macs, marcks, MGC89127, MARCKS, MGC137530 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Human (88), Rat (Rattus) (65), Mouse (Murine) (60), Cow (Bovine) (19), Dog (Canine) (18), Pig (Porcine) (18), Rabbit (17), Chicken (2), Guinea Pig (1), Mammalian (1), Zebrafish (Brachydanio rerio) (1)
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Application
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 Alternatives Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)
Western Blotting (WB) (72), Immunofluorescence (IF) (55), Flow Cytometry (FACS) (20), ELISA (13), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), Enzyme Immunoassay (EIA) (10), Immunohistochemistry (IHC) (9), Immunocytochemistry (ICC) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN240027 |
| Quantity |
100 µl (Variants) |
| Price |
446.67 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 10 to 15 Business Days |
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Alternative name
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MARCKS
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Swiss-Prot
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P29966
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Immunogen
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This antibody was made against full length recombinant MARCKS expressed in and purified from E. coli
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Description
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Gene: MARCKS, Standard Gene Symbol: MARCKS, Family: MARCKS, Synonyms: MARCKS, 80K-L, FLJ14368, FLJ90045, MACS, Myristoylated alanine-rich c-kinase substrate, PKCSL, PRKCSL
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Specificity
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The MARCKS protein runs at about 80kD on SDS-PAGE gels, although the real molecular is much lower, at about 31.5kD. The reason for this is probably because the protein is extremely rich in acidic residues. Such proteins appear to bind less SDS than the average protein, and so migrate in PAGE more slowly than the normal protein. MARCKS is a major protein of brain, and is concentrated in synapses of neurons. It appears to function in synaptic vesicle cycling and has been shown to bind both actin and calmodulin in vitro. MARCKS belongs to a family of proteins with similar actin and calmodulin binding properties. Deletion of the MARCKS gene in mice results in embryonic brain defects and death.
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Application Notes
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ICC (1:5000), IF (1:500 - 1:1000), IHC (1:5000), WB (1:20000)
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Purification
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Antiserum
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Buffer
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Serum.
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Storage
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Long term: -20°C, Short term: +4°C
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Research Area
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Cardiovascular
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Restrictions
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For Research Use only
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Alternatives for antigen "Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS)", type "Antibodies"
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Hosts
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Rabbit (96), Mouse (7), Rat (2)
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Reactivities
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Human (88), Rat (Rattus) (65), Mouse (Murine) (60), Cow (Bovine) (19), Dog (Canine) (18), Pig (Porcine) (18), Rabbit (17), Chicken (2), Guinea Pig (1), Mammalian (1), Zebrafish (Brachydanio rerio) (1)
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Applications
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Western Blotting (WB) (72), Immunofluorescence (IF) (55), Flow Cytometry (FACS) (20), ELISA (13), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), Enzyme Immunoassay (EIA) (10), Immunohistochemistry (IHC) (9), Immunocytochemistry (ICC) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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pSer158 (7), pSer162 (7), pSer170 (6), Ab-162 (4), C-Term (2), N-Term (2), pSer152, pSer156 (1), pSer152/156 (1), pSer163 (1), phospho (1)
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