ODF2 antibody (Outer Dense Fiber of Sperm Tails 2)

Details for Product anti-ODF2 Antibody No. ABIN2430583, Supplier: Log in to see
Antigen
  • odf84
  • odf2/1
  • odf2/2
  • DKFZp469J0214
  • CT134
  • ODF2/1
  • ODF2/2
  • ODF84
  • si:dkey-228b2.4
  • AI848335
  • MMTEST29
  • outer dense fiber of sperm tails 2
  • ODF2
  • odf2
  • Odf2
Alternatives
anti-Human ODF2 antibody for ELISA
Reactivity
Human, Mouse (Murine), Rat (Rattus)
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15
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2
1
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Host
Rabbit
60
14
3
1
Clonality
Polyclonal
Conjugate
This ODF2 antibody is un-conjugated
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3
3
2
2
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2
2
2
1
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1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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32
24
10
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5
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3
1
1
Options
Supplier
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Supplier Product No.
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'Independent Validation' Badge
Antigen Outer Dense Fiber of Sperm Tails 2 (ODF2)
Method validated Western Blotting
Positive Control recombinant ODF2-His, bacterially expressed; NIH3T3 total cell lysate
Notes Passed, regarding sensitivity and specificity. The antibody detects bacterially expressed ODF2 (see 2)) and the endogenous protein (see 3)) with only moderate background staining in WB.
Primary Antibody ABIN2430582
Secondary Antibody anti rabbit IgG (H+L), HRP-linked (Jackson Immuno Research, Code number: 111-035-003, Lot number: 123450)
Protocol
  • Proteins separated on 10% SDS-PAGE (Laemmli 1970), blotted to Protran (GE Healthcare No. 10600004, 0.2 µm) (Towbin et al., 1979). Blocking of membrane in 5% skim milk in TBST (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 0.1% Tween 20) for 30 min at room temperature.
  • Incubation with first antibody in 5% skim milk in TBST at 4°C overnight.
  • Washing in TBST for 30 minutes at room temperature.
  • Incubation with secondary antibody in 5% skim milk in TBST for 45 minutes at room temperature. Washing in TBST for 30-45 minutes at room temperature.
  • Secondary antibody: anti rabbit IgG (H+L), HRP-linked (Jackson Immuno Research, Code number: 111-035-003, Lot number: 123450). Dilution: 1:10,000.
  • Chemiluminescence detection (Western Lightning Ultra, Lot 215-15091, Perkin Elmer) and image capture via BioRad Chemdoc or X-ray films.
Validation Images
Western Blotting validation image for anti-Outer Dense Fiber of Sperm Tails 2 (ODF2) antibody (ABIN2430582) 1. Positive control: protein ODF2-His, bacterially expressed, different amounts per l...
'Independent Validation' Badge
Antigen Outer Dense Fiber of Sperm Tails 2 (ODF2)
Method validated Immunocytochemistry
Positive Control mouse anti-γ-tubulin (Sigma-Aldrich, clone GTU-88, T6557, lot number 072M4808)
Notes Passed, the antibody detects centrosomal ODF2 in immunocytological preparations with only very low background staining (the green dots are mostly the correct location of ODF2 as they overlap with γ-tubulin spots (in red)).
Primary Antibody ABIN2430582
Secondary Antibody MFP488 goat anti-rabbit IgG (H+L) (MoBiTec, #MFP-A1008)
Protocol
  • NIH3T3 cells (ATCC) were grown on cover slips in DMEM, 10% fetal bovine serum, 5% penicillin/streptomycin (all Gibco) at 37°C in 5% CO2.
  • Cells were fixed in ice-cold methanol for 20 min at -20°C.
  • Unspecific binding sites were blocked in PBST (phosphate buffered saline (PBS) containing 0.15% bovine serum albumin, 0.1% Tween-20) for 1 hr.
  • Cells were incubated with the primary antibodies at 4°C overnight.
  • Cells were washed in TBST (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 0.1% Tween 20) for 15 min.
  • Cells were incubated with secondary antibodies and DAPI for 1h at 37°C.
  • Cells were washed in TBST for 15 min.
  • Cells were embedded in Dako Fluorescent Mounting Medium (Dako).
  • As positive control for centrosomal decoration anti ?-tubulin (Sigma-Aldrich, clone GTU-88, T6557, lot number 072M4808; 1:100) was used. anti ?-tubulin was detected with MFP590 goat anti-mouse IgG (H+L) (MoBiTec, #MFP-A1032; 1:300).
  • Anti ODF2-antibody was detected with MFP488 goat anti-rabbit IgG (H+L) (MoBiTec, #MFP-A1008; 1:300).
  • DNA was counterstained with DAPI.
  • Images were taken by confocal microscopy (LSM 510, Zeiss), and processed using Adobe Photoshop 5.0.
Validation Images
Immunocytochemistry validation image for anti-Outer Dense Fiber of Sperm Tails 2 (ODF2) antibody (ABIN2430582) Anti-ODF2 antibody ABIN2430582 (ESAP15572) is detected with a MFP488 goat anti-rabbit...
Immunogen Recombinant protein of human ODF2
Isotype IgG
Purification Antigen affinity purification
Alternative Name ODF2 (ODF2 Antibody Abstract)
Background The outer dense fibers are cytoskeletal structures that surround the axoneme in the middle piece and principal piece of the sperm tail. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation. Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. This gene encodes one of the major outer dense fiber proteins. Alternative splicing results in multiple transcript variants.
Synonyms: CT134, ODF84, ODF2/1, ODF2/2
Molecular Weight Calculated MW: 95 kDa
Pathways M Phase
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid freeze / thaw cycles.
Storage -20 °C/-80 °C
Storage Comment Store at -20°C (regular) and -80°C (long term).
Supplier Images
Western Blotting (WB) image for anti-Outer Dense Fiber of Sperm Tails 2 (ODF2) antibody (ABIN2430583) Western blot analysis of Human testis tissue and K562 cell, using ODF2 Polyclonal An...
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