PPARG antibody (AA 82-101)
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- Target See all PPARG Antibodies
- PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))
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Binding Specificity
- AA 82-101
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Reactivity
- Human, Dog
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PPARG antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human PPAR gamma 1 amino acids 82-101 (PASPPYYSEKTQLYNKPHEE, amino acids 110-129 of PPAR gamma 2)1
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Cat, Dog, Elephant, Panda (100%) Marmoset, Mouse, Rat, Bat, Hamster, Rabbit, Pig, Opossum (95%) Sheep, Bovine, Goat, Horse (90%) Turkey, Chicken (85%) Platypus, Trachemys (80%).
- Purification
- Immunoaffinity purified
- Immunogen
- Human PPAR1 amino acids 82-101 (PASPPYYSEKTQLYNKPHEE). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Cat, Dog, Elephant, Panda (100%), Marmoset, Mouse, Rat, Bat, Hamster, Rabbit, Pig, Opossum (95%), Sheep, Bovine, Goat, Horse (90%), Turkey, Chicken (85%), Platypus, Trachemys (80%).
- Isotype
- IgG
- Top Product
- Discover our top product PPARG Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (5 μg/mL), WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- TBS, 50 % glycerol, 0.5 mg/mL BSA, 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))
- Alternative Name
- PPARG / PPAR Gamma (PPARG Products)
- Synonyms
- PPAR gamma antibody, PPARg2 antibody, PPARG antibody, Nr1c3 antibody, PPAR-gamma antibody, PPAR-gamma2 antibody, PPARgamma antibody, PPARgamma2 antibody, NR1C3 antibody, CIMT1 antibody, GLM1 antibody, PPARG1 antibody, PPARG2 antibody, xPPAR-gamma antibody, PPAR-GAMMA antibody, PPARGAMMA antibody, peroxisome proliferator-activated receptor gamma antibody, peroxisome proliferator activated receptor gamma antibody, peroxisome proliferator activated receptor gamma L homeolog antibody, PPARG antibody, Pparg antibody, pparg.L antibody
- Background
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Name/Gene ID: PPARG
Subfamily: NR1 Thyroid hormone-like
Family: NHR
Synonyms: PPARG, NR1C3, PPAR gamma, PPAR gamma 2, PPARG1, PPARG2, PPARgamma, CIMT1, GLM1, PPAR-gamma - Gene ID
- 5468
- UniProt
- P37231
- Pathways
- MAPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
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