GFP antibody (Green Fluorescent Protein)

Details for Product anti-GFP Antibody No. ABIN2451989, Supplier: Log in to see
Antigen
  • green fluorescent protein
  • gfp
Reactivity
Aequorea victoria
639
5
5
2
2
2
1
1
Host
Rabbit
328
170
80
41
16
9
8
2
Clonality
Polyclonal
Conjugate
This GFP antibody is un-conjugated
32
30
27
14
12
11
11
8
8
8
7
7
6
5
5
5
5
5
5
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
522
337
188
143
141
119
96
72
58
26
15
13
13
8
8
6
6
5
4
4
3
3
3
3
3
2
2
1
1
1
1
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Available images

Immunogen Recombinant His-tagged EGFP
Specificity Reactive to all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, EGFP, and their-fusion proteins
Characteristics Antiserum
Purification Rabbit serum
Alternative Name GFP
Background The green fluorescent protein (GFP) is composed of 238 amino acids (26.9 kDa), originally isolated from the jellyfish Aequorea victoria that fluoresces green when exposed to blue light. In cell and molecular biology, the GFP fused gene is frequently used as a reporter of expression and protein localization.
UniProt P42212
Research Area Tags/Labels
Application Notes 1. Western blotting: 1/2,000 dilution
2. Immunoprecipitation (assay dependent)
3. Immunohistochemistry: 1/4,000 dilution
4. Immunofluorescence staining (assay dependent)
Restrictions For Research Use only
Format Liquid
Buffer 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Upon receipt aliquot and store at -20 C
Supplier Images
Western Blotting (WB) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN2451989) anti-Green Fluorescent Protein (GFP) antibody
Western Blotting (WB) image for anti-GFP antibody (Green Fluorescent Protein) (ABIN2451989) anti-Green Fluorescent Protein (GFP) antibody (Image 2)
Background publications Tsien: "The green fluorescent protein." in: Annual review of biochemistry, Vol. 67, pp. 509-44, 1998 (PubMed).

Chalfie, Tu, Euskirchen, Ward, Prasher: "Green fluorescent protein as a marker for gene expression." in: Science (New York, N.Y.), Vol. 263, Issue 5148, pp. 802-5, 1994 (PubMed).

Product cited in: SHIMOMURA, JOHNSON, SAIGA: "Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea." in: Journal of cellular and comparative physiology, Vol. 59, pp. 223-39, 1970 (PubMed).