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IZUMO1 antibody

IZUMO1 Reactivity: Human, Mouse WB, IF, IP Host: Rat Monoclonal #125 unconjugated
Catalog No. ABIN2452040
  • Target See all IZUMO1 Antibodies
    IZUMO1 (Izumo Sperm-Egg Fusion 1 (IZUMO1))
    Reactivity
    Human, Mouse
    Host
    • 25
    Rat
    Clonality
    • 24
    Monoclonal
    Conjugate
    • 11
    • 4
    • 3
    • 3
    • 2
    • 2
    This IZUMO1 antibody is un-conjugated
    Application
    • 20
    • 20
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
    Cross-Reactivity (Details)
    Not tested in other species.
    Purification
    Purified
    Sterility
    Sterile filtered
    Immunogen
    Mouse sperm
    Clone
    #125
    Isotype
    IgG2a kappa
  • Application Notes
    1. Western blotting: 1/1,000 dilution
    2. Immunoprecipitation: 1/100 dilution
    3. Immunofluorescence staining: 1/250 dilution
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS, 50 % glycerol. Azide- and carrier protein-free.
    Preservative
    Azide free
    Storage
    -20 °C
    Storage Comment
    Upon arrival centrifuge briefly, aliquote and store at -20 C.
  • Barbaux, Ialy-Radio, Chalbi, Dybal, Homps-Legrand, Do Cruzeiro, Vaiman, Wolf, Ziyyat: "Sperm SPACA6 protein is required for mammalian Sperm-Egg Adhesion/Fusion." in: Scientific reports, Vol. 10, Issue 1, pp. 5335, (2020) (PubMed).

    Inoue, Hamada, Kamikubo, Hirata, Kataoka, Yamamoto, Ikawa, Okabe, Hagihara: "Molecular dissection of IZUMO1, a sperm protein essential for sperm-egg fusion." in: Development (Cambridge, England), Vol. 140, Issue 15, pp. 3221-9, (2013) (PubMed).

    Satouh, Inoue, Ikawa, Okabe: "Visualization of the moment of mouse sperm-egg fusion and dynamic localization of IZUMO1." in: Journal of cell science, Vol. 125, Issue Pt 21, pp. 4985-90, (2013) (PubMed).

    Ikawa, Tokuhiro, Yamaguchi, Benham, Tamura, Wada, Satouh, Inoue, Okabe: "Calsperin is a testis-specific chaperone required for sperm fertility." in: The Journal of biological chemistry, Vol. 286, Issue 7, pp. 5639-46, (2011) (PubMed).

    Inoue, Kasahara, Ikawa, Okabe: "Identification and disruption of sperm-specific angiotensin converting enzyme-3 (ACE3) in mouse." in: PLoS ONE, Vol. 5, Issue 4, pp. e10301, (2010) (PubMed).

  • Target
    IZUMO1 (Izumo Sperm-Egg Fusion 1 (IZUMO1))
    Alternative Name
    IZUMO1 (IZUMO1 Products)
    Synonyms
    IZUMO antibody, 1700058F15Rik antibody, Izumo antibody, izumo sperm-egg fusion 1 antibody, Ras interacting protein 1 antibody, IZUMO1 antibody, RASIP1 antibody, Izumo1 antibody
    Background
    Key words: Acrosome reaction, Membrane fusion, Protein trafficking, IZUMO1, Sperm-egg fusion
    Molecular Weight
    44,885 Da
    UniProt
    Q9D9J7, Q8IYV9
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