KEAP1 antibody

Catalog No. ABIN2724101

Product Details anti-KEAP1 Antibody

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This KEAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Characteristics: Homo sapiens kelch-like ECH-associated protein 1 (KEAP1), transcript variant 1

Purification: Purified from mouse ascites fluids by affinity chromatography

Immunogen: Full length human recombinnt protein of human KEAP1(NP_987096) produced in HEK293T cell.

Clone: 1B4

Isotype: IgG1

Application Details

Application Notes: WB 1:500~2000, IHC 1:150, FLOW 1:100,

Comment:

The concentration of the product may vary between diferrent lots.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5-1.0 mg/mL

Buffer: PBS (PH 7.3) containing 1 % BSA, 50 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-KEAP1 antibody (ABIN2724101)

Mulvaney, Matson, Siesser, Tamir, Goldfarb, Jacobs, Cloer, Harrison, Vaziri, Cook, Major: "Identification and Characterization of MCM3 as a Kelch-like ECH-associated Protein 1 (KEAP1) Substrate." in: The Journal of biological chemistry, Vol. 291, Issue 45, pp. 23719-23733, (2016) (PubMed).

Caner, Sen Turk, Baris, Cetin, Tepeli, Hacioglu, Sari, Zencir, Dogu, Bagci, Keskin: "MYD88 Expression and L265P Mutation in Mature B-Cell Non-Hodgkin Lymphomas." in: Genetic testing and molecular biomarkers, Vol. 19, Issue 7, pp. 372-8, (2015) (PubMed).

Mercado, Kizawa, Ueda, Xiong, Kimura, Moses, Curtis, Ito, Barnes: "Activation of transcription factor Nrf2 signalling by the sphingosine kinase inhibitor SKI-II is mediated by the formation of Keap1 dimers." in: PLoS ONE, Vol. 9, Issue 2, pp. e88168, (2014) (PubMed).

Michaeloudes, Mercado, Clarke, Bhavsar, Adcock, Barnes, Chung: "Bromodomain and extraterminal proteins suppress NF-E2-related factor 2-mediated antioxidant gene expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 192, Issue 10, pp. 4913-20, (2014) (PubMed).

Zhao, Duan, Yuan, Quan, Yang, Xi: "A reusable localized surface plasmon resonance biosensor for quantitative detection of serum squamous cell carcinoma antigen in cervical cancer patients based on silver nanoparticles array." in: International journal of nanomedicine, Vol. 9, pp. 1097-104, (2014) (PubMed).

Vieceli Dalla Sega, Zambonin, Fiorentini, Rizzo, Caliceti, Landi, Hrelia, Prata: "Specific aquaporins facilitate Nox-produced hydrogen peroxide transport through plasma membrane in leukaemia cells." in: Biochimica et biophysica acta, Vol. 1843, Issue 4, pp. 806-14, (2014) (PubMed).

Rondini, Fang, Runge-Morris, Kocarek: "Regulation of human cytosolic sulfotransferases 1C2 and 1C3 by nuclear signaling pathways in LS180 colorectal adenocarcinoma cells." in: Drug metabolism and disposition: the biological fate of chemicals, Vol. 42, Issue 3, pp. 361-8, (2014) (PubMed).

Adebiyi, Soni, John, Yang: "Lipid rafts are required for signal transduction by angiotensin II receptor type 1 in neonatal glomerular mesangial cells." in: Experimental cell research, Vol. 324, Issue 1, pp. 92-104, (2014) (PubMed).

Barnett, Liebl, Seymour, Gillen, Lim, Larock, Davies, Schulz, Nizet, Teasdale, Walker: "The globally disseminated M1T1 clone of group A Streptococcus evades autophagy for intracellular replication." in: Cell host & microbe, Vol. 14, Issue 6, pp. 675-82, (2013) (PubMed).

Tanaka, Tanaka, Harada, Kohda, Matsumura, Shimamoto, Sawabe, Marunaka, Kuwabara, Takahashi, Ito, Nakahari: "A PKG inhibitor increases Ca(2+)-regulated exocytosis in guinea pig antral mucous cells: cAMP accumulation via PDE2A inhibition." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 304, Issue 9, pp. G773-80, (2013) (PubMed).

Ramdzan, Polling, Chia, Ng, Ormsby, Croft, Purcell, Bogoyevitch, Ng, Gleeson, Hatters: "Tracking protein aggregation and mislocalization in cells with flow cytometry." in: Nature methods, Vol. 9, Issue 5, pp. 467-70, (2012) (PubMed).

Target Details for KEAP1

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Alternative Name: KEAP1 (KEAP1 Products)

Synonyms: INrf2 antibody, KLHL19 antibody, INRF2 antibody, mKIAA0132 antibody, Inrf2 antibody, fb36f11 antibody, keap1 antibody, wu:fb36f11 antibody, KEAP1 antibody, klhl19 antibody, zgc:171654 antibody, kelch like ECH associated protein 1 antibody, kelch-like ECH-associated protein 1 antibody, Kelch-like ECH-associated protein 1 antibody, kelch-like ECH-associated protein 1a antibody, kelch like ECH associated protein 1 L homeolog antibody, kelch-like ECH-associated protein 1b antibody, KEAP1 antibody, Keap1 antibody, keap1a antibody, keap1 antibody, keap1.L antibody, keap1b antibody

Background: This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.

Molecular Weight: 69.5 kDa

Gene ID: 9817

NCBI Accession: NM_203500

HGNC: 9817

Pathways: Maintenance of Protein Location