PURA antibody

Catalog No. ABIN2458010

Product Details anti-PURA Antibody

Target: PURA (Purine-Rich Element Binding Protein A (PURA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PURA antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PURA.

Application Details

Application Notes: PURA antibody can be used for detection of PURA by ELISA at 1:62500. PURA antibody can be used for detection of PURA by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store PURA antibody at -20 °C.

Target Details for PURA

Target: PURA (Purine-Rich Element Binding Protein A (PURA))

Alternative Name: PURA (PURA Products)

Synonyms: CAGER-1 antibody, Pur-alpha antibody, ssCRE-BP antibody, PUR-ALPHA antibody, PUR1 antibody, PURALPHA antibody, zgc:103411 antibody, pur-alpha antibody, pur1 antibody, puralpha antibody, purine-rich element binding protein A antibody, purine rich element binding protein A antibody, purine-rich element binding protein Aa antibody, purine-rich element binding protein A L homeolog antibody, pura antibody, Pura antibody, PURA antibody, puraa antibody, pura.L antibody

Background: PURA is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia.This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Molecular Weight: 35 kDa

Gene ID: 5813

NCBI Accession: NP_005850

UniProt: Q00577