MED6 antibody

Catalog No. ABIN2458077

Product Details anti-MED6 Antibody

Target: MED6 (Mediator Complex Subunit 6 (MED6))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MED6.

Application Details

Application Notes: MED6 antibody can be used for detection of MED6 by ELISA at 1:312500. MED6 antibody can be used for detection of MED6 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store MED6 antibody at -20 °C.

Target Details for MED6

Target: MED6 (Mediator Complex Subunit 6 (MED6))

Alternative Name: MED6 (MED6 Products)

Synonyms: NY-REN-28 antibody, wu:fa14c11 antibody, zgc:86933 antibody, 1500012F11Rik antibody, AU019660 antibody, AV213303 antibody, RNA polymerase transcriptional regulation mediator-like protein antibody, mediator complex subunit 6 antibody, hypothetical protein antibody, MED6 antibody, Med6 antibody, med6 antibody, CAALFM_C306170CA antibody

Background: MED6 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. And mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Molecular Weight: 28 kDa

Gene ID: 10001

NCBI Accession: NP_005457

UniProt: O75586

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha