SSX4 antibody

Catalog No. ABIN2458169

Product Details anti-SSX4 Antibody

Target: SSX4 (Synovial Sarcoma, X Breakpoint 4 (SSX4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SSX4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SSX4.

Application Details

Application Notes: SSX4 antibody can be used for detection of SSX4 by ELISA at 1:312500. SSX4 antibody can be used for detection of SSX4 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store SSX4 antibody at -20 °C.

Target Details for SSX4

Target: SSX4 (Synovial Sarcoma, X Breakpoint 4 (SSX4))

Alternative Name: SSX4 (SSX4 Products)

Synonyms: CT5.4 antibody, SSX family member 4 antibody, SSX4 antibody

Background: SSX4 belongs to the SSX family. It contains 1 KRAB-related domain.SSX4 could act as a modulator of transcription. The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. SSX1, SSX2 and SSX4 genes have been involved in the t (X,18) translocation characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. Chromosome Xp11 contains a segmental duplication resulting in two identical copies of synovial sarcoma, X breakpoint 4, SSX4 and SSX4B, in tail-to-tail orientation. This gene, SSX4, represents the more telomeric copy. Two transcript variants encoding distinct isoforms have been identified for this gene.

Molecular Weight: 22 kDa

Gene ID: 6759

NCBI Accession: NP_005627

UniProt: O60224