RAD54B antibody

Catalog No. ABIN2458241

Product Details anti-RAD54B Antibody

Target: RAD54B (DNA repair and recombination protein RAD54B (RAD54B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD54B antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RAD54B.

Application Details

Application Notes: RAD54B antibody can be used for detection of RAD54B by ELISA at 1:62500. RAD54B antibody can be used for detection of RAD54B by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store RAD54B antibody at -20 °C.

Target Details for RAD54B

Target: RAD54B (DNA repair and recombination protein RAD54B (RAD54B))

Alternative Name: RAD54B (RAD54B Products)

Synonyms: RAD54B antibody, im:7137737 antibody, im:7153525 antibody, fsbp antibody, rdh54 antibody, RDH54 antibody, E130016E03Rik antibody, Fsbp antibody, RGD1306507 antibody, RAD54 homolog B (S. cerevisiae) antibody, RAD54 homolog B L homeolog antibody, RAD54B antibody, rad54b antibody, rad54b.L antibody, Rad54b antibody

Background: RAD54B belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer.The protein encoded by this gene belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer.

Molecular Weight: 103 kDa

Gene ID: 25788

NCBI Accession: NP_036547

UniProt: Q9Y620