IREB2 antibody

Catalog No. ABIN2458508

Product Details anti-IREB2 Antibody

Target: IREB2 (Iron-Responsive Element Binding Protein 2 (IREB2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IREB2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human IREB2.

Application Details

Application Notes: IREB2 antibody can be used for detection of IREB2 by ELISA at 1:312500. IREB2 antibody can be used for detection of IREB2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store IREB2 antibody at -20 °C.

Target Details for IREB2

Target: IREB2 (Iron-Responsive Element Binding Protein 2 (IREB2))

Alternative Name: IREB2 (IREB2 Products)

Synonyms: IREB2 antibody, im:7153062 antibody, DKFZp468N047 antibody, D9Ertd85e antibody, Irp2 antibody, ACO3 antibody, IRP2 antibody, IRP2AD antibody, IREBP2 antibody, iron responsive element binding protein 2 antibody, iron-responsive element binding protein 2 antibody, iron responsive element binding protein 2 L homeolog antibody, IREB2 antibody, ireb2 antibody, Ireb2 antibody, ireb2.L antibody

Background: IREB2 binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. IREB2 binds to the IRE element in ferritin which results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA.

Molecular Weight: 105 kDa

Gene ID: 3658

NCBI Accession: NP_004127

UniProt: P48200

Pathways: Transition Metal Ion Homeostasis