PAPOLG antibody

Catalog No. ABIN2458544

Product Details anti-PAPOLG Antibody

Target: PAPOLG (Poly(A) Polymerase gamma (PAPOLG))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAPOLG antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PAPOLG.

Application Details

Application Notes: PAPOLG antibody can be used for detection of PAPOLG by ELISA at 1:1562500. PAPOLG antibody can be used for detection of PAPOLG by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store PAPOLG antibody at -20 °C.

Target Details for PAPOLG

Target: PAPOLG (Poly(A) Polymerase gamma (PAPOLG))

Alternative Name: PAPOLG (PAPOLG Products)

Synonyms: zgc:63762 antibody, 9630006B20Rik antibody, poly(A) polymerase gamma antibody, poly(A) polymerase gamma L homeolog antibody, papolg antibody, papolg.L antibody, PAPOLG antibody, Papolg antibody

Background: PAPOLG is a member of the poly (A) polymerase family which catalyzes template-independent extension of the 3' end of a DNA/RNA strand. This enzyme shares 60 % identity to the well characterized poly (A) polymerase II (PAPII) at the amino acid level. These two enzymes have similar organization of structural and functional domains. This enzyme is exclusively localized in the nucleus and exhibits both nonspecific and CPSF (cleavage and polyadenylation specificity factor)/AAUAAA-dependent polyadenylation activity.This gene encodes a member of the poly (A) polymerase family which catalyzes template-independent extension of the 3' end of a DNA/RNA strand. This enzyme shares 60 % identity to the well characterized poly (A) polymerase II (PAPII) at the amino acid level. These two enzymes have similar organization of structural and functional domains. This enzyme is exclusively localized in the nucleus and exhibits both nonspecific and CPSF (cleavage and polyadenylation specificity factor)/AAUAAA-dependent polyadenylation activity. This gene is located on chromosome 2 in contrast to the PAPII gene, which is located on chromosome 14.

Molecular Weight: 83 kDa

Gene ID: 64895

NCBI Accession: NP_075045

UniProt: Q9BWT3