Nucleolar Protein 6 antibody

Catalog No. ABIN2458545

Product Details anti-Nucleolar Protein 6 Antibody

Target: Nucleolar Protein 6 (NOL6)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nucleolar Protein 6 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NOL6.

Application Details

Application Notes: NOL6 antibody can be used for detection of NOL6 by ELISA at 1:1562500. NOL6 antibody can be used for detection of NOL6 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store NOL6 antibody at -20 °C.

Target Details for Nucleolar Protein 6

Target: Nucleolar Protein 6 (NOL6)

Alternative Name: NOL6 (NOL6 Products)

Synonyms: NRAP antibody, UTP22 antibody, bA311H10.1 antibody, nrap antibody, utp22 antibody, AA410091 antibody, Nrap antibody, nucleolar protein 6 antibody, nucleolar protein 6 L homeolog antibody, nucleolar protein family 6 (RNA-associated) antibody, NOL6 antibody, Nol6 antibody, nol6.L antibody

Background: NOL6 is a nucleolar RNA-associated protein that is highly conserved between species. RNase treatment of permeabilized cells indicates that the nucleolar localization is RNA dependent. Further studies suggest that the protein is associated with ribosome biogenesis through an interaction with pre-rRNA primary transcripts. The nucleolus is a dense subnuclear membraneless organelle that assembles around clusters of rRNA genes and functions in ribosome biogenesis. This gene encodes a nucleolar RNA-associated protein that is highly conserved between species. RNase treatment of permeabilized cells indicates that the nucleolar localization is RNA dependent. Further studies suggest that the protein is associated with ribosome biogenesis through an interaction with pre-rRNA primary transcripts. Alternative splicing has been observed at this locus and two splice variants encoding distinct isoforms have been identified.

Molecular Weight: 127 kDa

Gene ID: 65083

NCBI Accession: NP_075068

UniProt: Q9H6R4