MARCH7
Reactivity: Human, Mouse, Rat
WB, IF (p)
Host: Rabbit
Polyclonal
Alexa Fluor 488
Application Notes
MARCH7 antibody can be used for detection of 40244 by ELISA at 1:62500. MARCH7 antibody can be used for detection of 40244 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Concentration
1 mg/mL
Buffer
Antibody is lyophilized in PBS buffer with 2 % sucrose.
Handling Advice
As with any antibody avoid repeat freeze-thaw cycles.
Storage
4 °C/-20 °C
Storage Comment
For short periods of storage (days) store at 4 °C. For longer periods of storage, store MARCH7 antibody at -20 °C.
Target
MARCH7
(Membrane-Associated Ring Finger (C3HC4) 7, E3 Ubiquitin Protein Ligase (MARCH7))
MARCH7 is an E3 ubiquitin-protein ligase which may specifically enhance the E2 activity of HIP2. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.