ATP5F1 antibody

Catalog No. ABIN2459178

Product Details anti-ATP5F1 Antibody

Target: ATP5F1 (ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit B1 (ATP5F1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP5F1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATP5F1.

Application Details

Application Notes: ATP5F1 antibody can be used for detection of ATP5F1 by ELISA at 1:12500. ATP5F1 antibody can be used for detection of ATP5F1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store ATP5F1 antibody at -20 °C.

Target Details for ATP5F1

Target: ATP5F1 (ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit B1 (ATP5F1))

Alternative Name: ATP5F1 (ATP5F1 Products)

Synonyms: PIG47 antibody, C76477 antibody, wu:fb59g11 antibody, wu:fj08b08 antibody, zgc:101887 antibody, ATP synthase, H+ transporting, mitochondrial Fo complex subunit B1 antibody, ATP synthase, H+ transporting, mitochondrial Fo complex subunit B1 S homeolog antibody, ATP synthase F(0) complex subunit B1, mitochondrial antibody, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1 antibody, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit B1 antibody, ATP5F1 antibody, atp5f1.S antibody, atp5f1 antibody, LOC479901 antibody, Atp5f1 antibody

Background: ATP5F1 is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the b subunit of the proton channel.This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the b subunit of the proton channel.

Molecular Weight: 25 kDa

Gene ID: 515

NCBI Accession: NP_001679

UniProt: P24539

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process