TRMT5 antibody

Catalog No. ABIN2459241

Product Details anti-TRMT5 Antibody

Target: TRMT5 (tRNA Methyltransferase 5 (TRMT5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRMT5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TRMT5.

Application Details

Application Notes: TRMT5 antibody can be used for detection of TRMT5 by ELISA at 1:62500. TRMT5 antibody can be used for detection of TRMT5 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store TRMT5 antibody at -20 °C.

Target Details for TRMT5

Target: TRMT5 (tRNA Methyltransferase 5 (TRMT5))

Alternative Name: TRMT5 (TRMT5 Products)

Synonyms: RGD1306567 antibody, 2610027O18Rik antibody, mKIAA1393 antibody, wu:fi28b08 antibody, KIAA1393 antibody, TRM5 antibody, tRNA methyltransferase 5 antibody, TRM5 tRNA methyltransferase 5 antibody, TRM5 tRNA methyltransferase 5 homolog (S. cerevisiae) antibody, Trmt5 antibody, TRMT5 antibody, trmt5 antibody

Background: TRNAs contain as many as 13 or 14 nucleotides that are modified posttranscriptionally by enzymes that are highly specific for particular nucleotides in the tRNA structure. TRMT5 methylates the N1 position of guanosine-37 (G37) in selected tRNAs using S-adenosyl methionine.tRNAs contain as many as 13 or 14 nucleotides that are modified posttranscriptionally by enzymes that are highly specific for particular nucleotides in the tRNA structure. TRMT5 methylates the N1 position of guanosine-37 (G37) in selected tRNAs using S-adenosyl methionine (Brule et al., 2004 [PubMed 15248782]).

Molecular Weight: 58 kDa

Gene ID: 57570

NCBI Accession: NP_065861

UniProt: Q32P41