EIF5 antibody

Catalog No. ABIN2459677

Product Details anti-EIF5 Antibody

Target: EIF5 (Eukaryotic Translation Initiation Factor 5 (EIF5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF5.

Application Details

Application Notes: EIF5 antibody can be used for detection of EIF5 by ELISA at 1:312500. EIF5 antibody can be used for detection of EIF5 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store EIF5 antibody at -20 °C.

Target Details for EIF5

Target: EIF5 (Eukaryotic Translation Initiation Factor 5 (EIF5))

Alternative Name: EIF5 (EIF5 Products)

Synonyms: fb37c12 antibody, fb54h04 antibody, zgc:56606 antibody, zgc:77026 antibody, wu:fb37c12 antibody, wu:fb54h04 antibody, CG9177 antibody, Dmel\\CG9177 antibody, EIf5 antibody, anon-EST:fe3C6 antibody, eIF-5 antibody, EIF-5 antibody, EIF-5A antibody, 2810011H21Rik antibody, D12Ertd549e antibody, eukaryotic translation initiation factor 5 antibody, CG9177 gene product from transcript CG9177-RD antibody, eukaryotic translation initiation factor 5 S homeolog antibody, Eukaryotic initiation factor-5 antibody, eif5 antibody, EIF5 antibody, eIF5 antibody, eif5.S antibody, Eif5 antibody

Background: EIF5 catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).

Molecular Weight: 49 kDa

Gene ID: 1983

NCBI Accession: NP_892116

UniProt: P55010