NFX1 antibody

Catalog No. ABIN2460119

Product Details anti-NFX1 Antibody

Target: NFX1 (Nuclear Transcription Factor, X-Box Binding 1 (NFX1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFX1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFX1.

Application Details

Application Notes: NFX1 antibody can be used for detection of NFX1 by ELISA at 1:312500. NFX1 antibody can be used for detection of NFX1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store NFX1 antibody at -20 °C.

Target Details for NFX1

Target: NFX1 (Nuclear Transcription Factor, X-Box Binding 1 (NFX1))

Alternative Name: NFX1 (NFX1 Products)

Synonyms: NFX1 antibody, NFX2 antibody, 1300017N15Rik antibody, 3000003M19Rik antibody, NFX.1 antibody, TEG-42 antibody, Tex42 antibody, nuclear transcription factor, X-box binding 1 antibody, nuclear transcription factor, x-box binding 1 antibody, NFX1 antibody, CpipJ_CPIJ011986 antibody, nfx1 antibody, Nfx1 antibody

Background: MHC class II gene expression is controlled primarily at the transcriptional level by transcription factors that bind to the X and Y boxes, two highly conserved elements in the proximal promoter of MHC class II genes. NFX1 is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. The protein may play a role in regulating the duration of an inflammatory response by limiting the period in which class II MHC molecules are induced by IFN-gamma.MHC class II gene expression is controlled primarily at the transcriptional level by transcription factors that bind to the X and Y boxes, two highly conserved elements in the proximal promoter of MHC class II genes. The protein encoded by this gene is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. The protein may play a role in regulating the duration of an inflammatory response by limiting the period in which class II MHC molecules are induced by IFN-gamma. Three alternative splice variants, each of which encodes a different isoform, have been identified.

Molecular Weight: 124 kDa

Gene ID: 4799

NCBI Accession: NP_002495

UniProt: Q12986