POU1F1 antibody

Catalog No. ABIN2460215

Product Details anti-POU1F1 Antibody

Target: POU1F1 (POU Class 1 Homeobox 1 (POU1F1))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POU1F1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POU1F1.

Application Details

Application Notes: POU1F1 antibody can be used for detection of POU1F1 by ELISA at 1:12500. POU1F1 antibody can be used for detection of POU1F1 by western blot at 1.0-10.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store POU1F1 antibody at -20 °C.

Target Details for POU1F1

Target: POU1F1 (POU Class 1 Homeobox 1 (POU1F1))

Alternative Name: POU1F1 (POU1F1 Products)

Synonyms: CPHD1 antibody, GHF-1 antibody, PIT1 antibody, POU1F1a antibody, Pit-1 antibody, Hmp1 antibody, Pit1 antibody, Pit1-rs1 antibody, dw antibody, dwarf antibody, GHF1 antibody, GHF1A antibody, PIT1Z antibody, PIT-1 antibody, Pit-1* antibody, pit1 antibody, pit-1 antibody, POU class 1 homeobox 1 antibody, POU domain, class 1, transcription factor 1 antibody, POU1F1 antibody, Pou1f1 antibody, pou1f1 antibody

Background: PIT1 is a pituitary-specific transcription factor responsible for pituitary development and hormone expression in mammals and is a member of the POU family of transcription factors that regulate mammalian development. The POU family is so named because the first 3 members identified were PIT1 and OCT1 of mammals, and Unc-86 of C. elegans. PIT1 contains 2 protein domains, termed POU-specific and POU-homeo, which are both necessary for high affinity DNA binding on genes encoding growth hormone and prolactin. PIT1 is also important for regulation of the genes encoding prolactin and thyroid-stimulating hormone beta subunit by thyrotropin-releasing hormone and cyclic AMP.

Molecular Weight: 33 kDa

Gene ID: 5449

NCBI Accession: NP_000297

UniProt: P28069

Pathways: Regulation of Carbohydrate Metabolic Process