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ALF antibody

Reactivity: Human WB, ELISA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN2460590
  • Target
    ALF
    Reactivity
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 2
    • 2
    Rabbit
    Clonality
    • 2
    • 1
    Polyclonal
    Conjugate
    • 4
    Un-conjugated
    Application
    • 4
    • 3
    • 1
    Western Blotting (WB), ELISA
    Purification
    Antibody is purified by protein A chromatography method.
    Immunogen
    Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ALF.
  • Application Notes
    ALF antibody can be used for detection of ALF by ELISA at 1:1562500. ALF antibody can be used for detection of ALF by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.
    Concentration
    1 mg/mL
    Buffer
    Antibody is lyophilized in PBS buffer with 2 % sucrose.
    Handling Advice
    As with any antibody avoid repeat freeze-thaw cycles.
    Storage
    4 °C/-20 °C
    Storage Comment
    For short periods of storage (days) store at 4 °C. For longer periods of storage, store ALF antibody at -20 °C.
  • Target
    ALF
    Background
    The assembly and stability of the RNA polymerase II transcription pre-initiation complex on a eukaryotic core promoter involve the effects of TFIIA on the interaction between TATA-binding protein (TBP) and DNA. This gene encodes a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that is able to stabilize the binding of TBP to DNA and may be uniquely important to testis biology.
    Molecular Weight
    50 kDa
    Gene ID
    11036
    NCBI Accession
    NP_751946
    UniProt
    Q9UNN4
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