MRPS15 antibody

Catalog No. ABIN2461942

Product Details anti-MRPS15 Antibody

Target: MRPS15 (Mitochondrial Ribosomal Protein S15 (MRPS15))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPS15 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MRPS15.

Application Details

Application Notes: MRPS15 antibody can be used for detection of MRPS15 by ELISA at 1:62500. MRPS15 antibody can be used for detection of MRPS15 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store MRPS15 antibody at -20 °C.

Target Details for MRPS15

Target: MRPS15 (Mitochondrial Ribosomal Protein S15 (MRPS15))

Alternative Name: MRPS15 (MRPS15 Products)

Synonyms: CG4207 antibody, DmMRPS15 antibody, Dmel\\CG4207 antibody, MRP-S15 antibody, mRpS15 antibody, zgc:92862 antibody, MPR-S15 antibody, RPMS15 antibody, S15mt antibody, 1500003E24Rik antibody, 2410002B11Rik antibody, Mprs15 antibody, CG4207 gene product from transcript CG4207-RA antibody, mitochondrial ribosomal protein S15 antibody, mitochondrial ribosomal protein S15 L homeolog antibody, bonsai antibody, mrps15 antibody, MRPS15 antibody, mrpS15 antibody, mrps15.L antibody, Mrps15 antibody

Background: Mammalian mitochondrial ribosomal proteins help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRPS15 is a 28S subunit protein that belongs to the ribosomal protein S15P family. The protein is more than two times the size of its E. coli counterpart, with the 12S rRNA binding sites conserved. Between human and mouse, the protein is the least conserved among small subunit ribosomal proteins.Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S15P family. The encoded protein is more than two times the size of its E. coli counterpart, with the 12S rRNA binding sites conserved. Between human and mouse, the encoded protein is the least conserved among small subunit ribosomal proteins. Pseudogenes corresponding to this gene are found on chromosomes 15q and 19q.

Molecular Weight: 30 kDa

Gene ID: 64960

NCBI Accession: NP_112570

UniProt: P82914