NXF3 antibody

Catalog No. ABIN2462273

Product Details anti-NXF3 Antibody

Target: NXF3 (Nuclear RNA Export Factor 3 (NXF3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NXF3 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NXF3.

Application Details

Application Notes: NXF3 antibody can be used for detection of NXF3 by ELISA at 1:1562500. NXF3 antibody can be used for detection of NXF3 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store NXF3 antibody at -20 °C.

Target Details for NXF3

Target: NXF3 (Nuclear RNA Export Factor 3 (NXF3))

Alternative Name: NXF3 (NXF3 Products)

Synonyms: NXF3 antibody, Gm384 antibody, RGD1564923 antibody, nuclear RNA export factor 3 antibody, NXF3 antibody, LOC523530 antibody, LOC100060140 antibody, LOC100060240 antibody, LOC785244 antibody, Nxf3 antibody

Background: NXF3 is one member of a family of nuclear RNA export factors. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. NXF3 has shortened LRR and ubiquitin-associated domains and its RDB is unable to bind RNA. It is located in the nucleoplasm but is not associated with either the nuclear envelope or the nucleolus.This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene has shortened LRR and ubiquitin-associated domains and its RDB is unable to bind RNA. It is located in the nucleoplasm but is not associated with either the nuclear envelope or the nucleolus.

Molecular Weight: 58 kDa, 72 kDa, 60 kDa, 72 kDa, 72 kDa, 72 kDa, 72 kDa, 28 kDa

Gene ID: 56000

NCBI Accession: NP_071335

UniProt: Q9H4D5