ALAD antibody

Catalog No. ABIN2462445

Product Details anti-ALAD Antibody

Target: ALAD (Aminolevulinate Dehydratase (ALAD))

Reactivity: Human, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALAD antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ALAD.

Application Details

Application Notes: ALAD antibody can be used for detection of ALAD by ELISA at 1:312500. ALAD antibody can be used for detection of ALAD by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store ALAD antibody at -20 °C.

Target Details for ALAD

Target: ALAD (Aminolevulinate Dehydratase (ALAD))

Alternative Name: ALAD (ALAD Products)

Synonyms: DDBDRAFT_0190269 antibody, DDBDRAFT_0231415 antibody, DDB_0190269 antibody, DDB_0231415 antibody, ALAD antibody, ncf antibody, ALADH antibody, PBGS antibody, ALADR antibody, aminolevulinatedelta-dehydratase antibody, zgc:110219 antibody, Lv antibody, delta-aminolevulinate dehydratase antibody, aminolevulinate dehydratase antibody, delta-aminolevulinic acid dehydratase antibody, Delta-aminolevulinic acid dehydratase antibody, aminolevulinate dehydratase L homeolog antibody, aminolevulinate, delta-, dehydratase antibody, hemB antibody, ALAD antibody, PBGS antibody, STY0404 antibody, SAS1597 antibody, SACI_RS03725 antibody, PAAG_00299 antibody, VDBG_00315 antibody, HPPC_00830 antibody, MGYG_01952 antibody, hem2 antibody, alad.L antibody, Alad antibody, alad antibody

Background: The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 Molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway, zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria.The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 Molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway, zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. Alternatively spliced transcript variants encoding different isoforms have been identified.

Molecular Weight: 39 kDa, 37 kDa

Gene ID: 210

NCBI Accession: NP_001003945

UniProt: Q6ZMU0