ZP2 antibody

Catalog No. ABIN2462854

Product Details anti-ZP2 Antibody

Target: ZP2 (Zona Pellucida Glycoprotein 2 (ZP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ZP2.

Application Details

Application Notes: ZP2 antibody can be used for detection of ZP2 by ELISA at 1:62500. ZP2 antibody can be used for detection of ZP2 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store ZP2 antibody at -20 °C.

Target Details for ZP2

Target: ZP2 (Zona Pellucida Glycoprotein 2 (ZP2))

Alternative Name: ZP2 (ZP2 Products)

Synonyms: fa12e07 antibody, wu:fa12e07 antibody, zgc:152728 antibody, zp2 antibody, Zp-2 antibody, ZPA antibody, zona pellucida glycoprotein 2, tandem duplicate 5 antibody, zona pellucida glycoprotein 2 antibody, zp2.5 antibody, Zp2 antibody, ZP2 antibody

Background: The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. ZP2 is a structural component of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies.The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies.

Molecular Weight: 68 kDa

Gene ID: 7783

NCBI Accession: NP_003451

UniProt: Q05996