DPH1 antibody
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- Target See all DPH1 Antibodies
- DPH1 (DPH1 Homolog (DPH1))
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Reactivity
- Human, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This DPH1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Purification
- Antibody is purified by peptide affinity chromatography method.
- Immunogen
- Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DPH1.
- Top Product
- Discover our top product DPH1 Primary Antibody
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- Application Notes
- DPH1 antibody can be used for detection of DPH1 by ELISA at 1:312500. DPH1 antibody can be used for detection of DPH1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
- Concentration
- 1 mg/mL
- Buffer
- Antibody is lyophilized in PBS buffer with 2 % sucrose.
- Handling Advice
- As with any antibody avoid repeat freeze-thaw cycles.
- Storage
- 4 °C/-20 °C
- Storage Comment
- For short periods of storage (days) store at 4 °C. For longer periods of storage, store DPH1 antibody at -20 °C.
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- Target
- DPH1 (DPH1 Homolog (DPH1))
- Alternative Name
- DPH1 (DPH1 Products)
- Synonyms
- DPH1 antibody, DPH1-OVCA2 antibody, dph1-ovca2 antibody, dph2l antibody, dph2l1 antibody, ovca1 antibody, DPH2L antibody, DPH2L1 antibody, OVCA1 antibody, zgc:110702 antibody, 2310011M22Rik antibody, 4930488F09Rik antibody, AW551873 antibody, Dph2l1 antibody, Ovca1 antibody, RGD1562694 antibody, diphthamide biosynthesis 1 antibody, diphthamide biosynthesis protein 1 antibody, diphthamide biosynthesis 1 L homeolog antibody, 2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 antibody, DPH1 antibody, NCU08503 antibody, ATEG_01183 antibody, LELG_00973 antibody, HCAG_03806 antibody, AOR_1_728014 antibody, PTRG_05729 antibody, CTRG_01524 antibody, UREG_06411 antibody, BDBG_05574 antibody, MCYG_04396 antibody, PITG_12187 antibody, MGYG_02224 antibody, LOC100284663 antibody, dph1.L antibody, dph1 antibody, Dph1 antibody, dph-1 antibody
- Background
- Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2, MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]). Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2, MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).
- Molecular Weight
- 49 kDa
- Gene ID
- 1801
- NCBI Accession
- NP_001374
- UniProt
- Q9BZG8
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