COPB1 antibody

Catalog No. ABIN2463511

Product Details anti-COPB1 Antibody

Target: COPB1 (Coatomer Protein Complex, Subunit beta 1 (COPB1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COPB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human COPB1.

Application Details

Application Notes: COPB1 antibody can be used for detection of COPB1 by ELISA at 1:12500. COPB1 antibody can be used for detection of COPB1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store COPB1 antibody at -20 °C.

Target Details for COPB1

Target: COPB1 (Coatomer Protein Complex, Subunit beta 1 (COPB1))

Alternative Name: COPB1 (COPB1 Products)

Background: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins, the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.

Molecular Weight: 107 kDa

Gene ID: 1315

NCBI Accession: NP_057535

UniProt: P53618