DNAJC24 antibody

Catalog No. ABIN2463559

Product Details anti-DNAJC24 Antibody

Target: DNAJC24 (DnaJ (Hsp40) Homolog, Subfamily C, Member 24 (DNAJC24))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNAJC24 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DPH4.

Application Details

Application Notes: DPH4 antibody can be used for detection of DPH4 by ELISA at 1:312500. DPH4 antibody can be used for detection of DPH4 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store DPH4 antibody at -20 °C.

Target Details for DNAJC24

Target: DNAJC24 (DnaJ (Hsp40) Homolog, Subfamily C, Member 24 (DNAJC24))

Alternative Name: DPH4 (DNAJC24 Products)

Synonyms: DPH4 antibody, JJJ3 antibody, ZCSL3 antibody, 1700030A21Rik antibody, 2610027M02Rik antibody, AV066965 antibody, AW240712 antibody, Dph4 antibody, MmDjC7 antibody, Zcsl3 antibody, RGD1564710 antibody, DnaJ heat shock protein family (Hsp40) member C24 antibody, DNAJC24 antibody, Dnajc24 antibody

Background: Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2, MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH4 is 1 of several enzymes involved in synthesis of diphthamide in EEF2.Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2, MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH4 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).

Molecular Weight: 17 kDa

Gene ID: 120526

NCBI Accession: NP_859057

UniProt: Q6P3W2