NAPA antibody

Catalog No. ABIN2463756

Product Details anti-NAPA Antibody

Target: NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAPA antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Purification: Antibody is purified by Protein A/G affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NAPA.

Application Details

Application Notes: NAPA antibody can be used for detection of NAPA by ELISA at 1:75000. NAPA antibody can be used for detection of NAPA by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store NAPA antibody at -20 °C.

Target Details for NAPA

Target: NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))

Alternative Name: NAPA (NAPA Products)

Synonyms: SNAPA antibody, wu:fa26c04 antibody, zgc:63906 antibody, napb antibody, 1500039N14Rik antibody, AW209189 antibody, RA81 antibody, SNARE antibody, a-SNAP antibody, hyh antibody, alpha-SNAP antibody, Alpha-SNAP antibody, NSF attachment protein alpha antibody, N-ethylmaleimide-sensitive factor attachment protein, alpha b antibody, NSF attachment protein alpha L homeolog antibody, N-ethylmaleimide-sensitive factor attachment protein, alpha antibody, N-ethylmaleimide sensitive fusion protein attachment protein alpha antibody, soluble NSF attachment protein antibody, NAPA antibody, napab antibody, napa.L antibody, napa antibody, EHI_000300 antibody, Napa antibody, LOC102577508 antibody

Background: The 'SNARE hypothesis' is a model explaining the process of docking and fusion of vesicles to their target membranes. According to this model, membrane proteins from the vesicle (v-SNAREs) and proteins from the target membrane (t-SNAREs) govern the specificity of vesicle targeting and docking through mutual recognition. Once the 2 classes of SNAREs bind to each other, they form a complex that recruits the general elements of the fusion apparatus, namely NSF (N-ethylmaleimide-sensitive factor) and SNAPs (soluble NSF-attachment proteins), to the site of membrane fusion, thereby forming the 20S fusion complex. Alpha- and gamma-SNAP are found in a wide range of tissues and act synergistically in intra-Golgi transport. The sequence of the predicted 295-amino acid human protein encoded by NAPA shares 37 % , 60 %, and 67 % identity with the sequences of yeast, Drosophila, and squid alpha-SNAP, respectively.

Molecular Weight: 33 kDa

Gene ID: 8775

NCBI Accession: NP_003818

UniProt: P54920

Pathways: Synaptic Vesicle Exocytosis, Asymmetric Protein Localization