GTF2E1 antibody

Catalog No. ABIN2463845

Product Details anti-GTF2E1 Antibody

Target: GTF2E1 (General Transcription Factor IIE, Polypeptide 1, alpha 56kDa (GTF2E1))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GTF2E1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GTF2E1.

Application Details

Application Notes: GTF2E1 antibody can be used for detection of GTF2E1 by ELISA at 1:1562500. GTF2E1 antibody can be used for detection of GTF2E1 by western blot at 0.2 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store GTF2E1 antibody at -20 °C.

Target Details for GTF2E1

Target: GTF2E1 (General Transcription Factor IIE, Polypeptide 1, alpha 56kDa (GTF2E1))

Alternative Name: GTF2E1 (GTF2E1 Products)

Background: The general transcription factor TFIIE has an essential role in eukaryotic transcription initiation together with RNA polymerase II and other general factors. Human TFIIE consists of two subunits of relative molecular mass 57,000 (TFIIE-alpha) and 34,000 (TFIIE-beta) and joins the preinitiation complex after RNA polymerase II and TFIIF. TFIIE-alpha is necessary for transcription initiation together with TFIIE-beta, and recombinant TFIIE-alpha can fully replace the natural subunit in an in vitro transcription assay. Its sequence contains several interesting structural motifs (leucine repeat, zinc finger and helix-turn-helix) and sequence similarities to bacterial sigma factors that suggest direct involvement in the regulation of transcription initiation

Molecular Weight: 49 kDa

Gene ID: 2960

NCBI Accession: NP_005504

UniProt: P29083