SUB1 antibody

Catalog No. ABIN2463865

Product Details anti-SUB1 Antibody

Target: SUB1 (Activated RNA Polymerase II Transcriptional Coactivator p15 (SUB1))

Reactivity: Human, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PC4.

Application Details

Application Notes: PC4 antibody can be used for detection of PC4 by ELISA at 1:1562500. PC4 antibody can be used for detection of PC4 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store PC4 antibody at -20 °C.

Target Details for SUB1

Target: SUB1 (Activated RNA Polymerase II Transcriptional Coactivator p15 (SUB1))

Alternative Name: PC4 (SUB1 Products)

Synonyms: P15 antibody, PC4 antibody, p14 antibody, AI842364 antibody, P9 antibody, Pc4 antibody, Rpo2tc1 antibody, SUB1 homolog, transcriptional regulator antibody, SUB1 homolog (S. cerevisiae) antibody, SUB1 antibody, Sub1 antibody

Background: PC4 is a transcriptional coactivator. General transcription factor IIH protects promoters from PC4-mediated repression by relieving the topological constraint imposed by PC4 through the ERCC3 helicase activity rather than by reducing the repressive activity of PC4 via its phosphorylation.

Molecular Weight: 14 kDa

Gene ID: 10923

NCBI Accession: NP_006704

UniProt: P53999