H1FOO antibody

Catalog No. ABIN2463933

Product Details anti-H1FOO Antibody

Target: H1FOO (H1 Histone Family, Member O, Oocyte-Specific (H1FOO))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This H1FOO antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human H1FOO.

Application Details

Application Notes: H1FOO antibody can be used for detection of H1FOO by ELISA at 1:312500. H1FOO antibody can be used for detection of H1FOO by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store H1FOO antibody at -20 °C.

Target Details for H1FOO

Target: H1FOO (H1 Histone Family, Member O, Oocyte-Specific (H1FOO))

Alternative Name: H1FOO (H1FOO Products)

Synonyms: osH1 antibody, C86609 antibody, H1fo antibody, H1oo antibody, b4 antibody, RGD1566236 antibody, H1 histone family member O oocyte specific antibody, H1 histone family, member O, oocyte-specific antibody, H1 histone family member O oocyte specific S homeolog antibody, H1FOO antibody, H1foo antibody, h1foo.S antibody

Background: H1FOO may play a key role in the control of gene expression during oogenesis and early embryogenesis, presumably through the perturbation of chromatin structure. H1FOO is essential for meiotic maturation of germinal vesicle-stage oocytes. The somatic type linker histone H1c is rapidly replaced by H1oo in a donor nucleus transplanted into an oocyte. The greater mobility of H1oo as compared to H1c may contribute to this rapid replacement and increased instability of the embryonic chromatin structure. The rapid replacement of H1c with H1oo may play an important role in nuclear remodeling.Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The protein encoded is a member of the histone H1 family. This gene contains introns, unlike most histone genes. The protein encoded is a member of the histone H1 family. The related mouse gene is expressed only in oocytes.

Molecular Weight: 36 kDa

Gene ID: 132243

NCBI Accession: NP_722575

UniProt: Q8IZA3