This antibody is expected to recognize both reported isoforms (NP_004150.1, NP_683720.2).
Purification
LMP7 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
LMP7 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of LMP7.
PSMB8
Reactivity: Human
WB, IF
Host: Mouse
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 22 kDa band observed in human liver, lung and lymph node lysates (calculated MW of 29.8 kDa according to precursor NP_004150.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Schwarz et al, J Immunol. 2000 Jul 15,165 (2) :768-78. PMID: 10878350. Recommended concentration: 0.01-0.03 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.