MC5R antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
MC5R antibody was raised against a 12 amino acid synthetic peptide near the N-Terminus (near) of MC5R (near).
MC5R
Reactivity: Human
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 37 kDa band observed in human and mouse skeletal muscle lysates (calculated MW of 36.6 kDa according to NP_005904.1). Recommended concentration: 0.3-1 μg/mL. an additional band of unknown identity was also consistently observed at 30 kDa . This band was successfully blocked by incubation with the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.