Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Purification
IREB2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
IREB2 antibody was raised against a 15 amino acid synthetic peptide near the internal region of IREB2.
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 110 kDa band observed in human liver lysates (calculated MW of 105 kDa according to NP_004127.1). Recommended concentration: 1-3 μg/mL. an additional band of unknown identity was also consistently observed at 60 kDa . This band was successfully blocked by incubation with the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
IREB2
(Iron-Responsive Element Binding Protein 2 (IREB2))