Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Specificity
This antibody is expected to recognize both reported isoforms (NP_002947.1, NP_937883.1).
Purification
CLIP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
CLIP1 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of CLIP1.
ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 160 kDa band observed in human breast tumor lysates (calculated MW of 161 kDa according to NP_002947.1. Recommended concentration: 1-3 μg/mL. Immunohistochemistry: Immunohistochemistry: In paraffin embedded human thyroid gland shows structured cytoplasm staining in active epithelial cells. Recommended concentration, 2-4 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
CLIP1
(CAP-GLY Domain Containing Linker Protein 1 (CLIP1))