This antibody is expected to recognize both reported isoforms (NP_005210.3, NP_001073280.1).
Purification
DIAPH1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
DIAPH1 antibody was raised against an 11 amino acid synthetic peptide near the internal region of DIAPH1.
DIAPH1
Reactivity: Human
WB, ELISA
Host: Mouse
Monoclonal
1A8
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 140 kDa band observed in lysates of cell line NIH3T3 (calculated MW of 141 kDa according to human NP_005210.3 and 139 kDa according to mouse NP_031884.1). Recommended concentration: 0.3-1 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.