SPAG16 antibody (Internal Region)

Catalog No. ABIN2464413

Product Details anti-SPAG16 Antibody

Target: SPAG16 (Sperm Associated Antigen 16 (SPAG16))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SPAG16 antibody is un-conjugated

Application: ELISA

Specificity: This antibody is expected to recognize isoform 1 (NP_078808.3) only.

Purification: SPAG16 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: SPAG16 antibody was raised against a 12 amino acid synthetic peptide near the Internal region of SPAG16.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 55 kDa and 22 kDa in human testis lysates after 0.3 μg/mL antibody staining.8 kDa according to NP_078808.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for SPAG16

Target: SPAG16 (Sperm Associated Antigen 16 (SPAG16))

Alternative Name: SPAG16 (SPAG16 Products)

Synonyms: PF20 antibody, WDR29 antibody, 4921511D23Rik antibody, 4930524F24Rik antibody, 4930585K05Rik antibody, AV261009 antibody, Pf20 antibody, Wdr29 antibody, RGD1565062 antibody, sperm associated antigen 16 antibody, sperm-associated antigen 16 protein antibody, SPAG16 antibody, LOC694114 antibody, Spag16 antibody

Gene ID: 79582

NCBI Accession: NP_078808

UniProt: Q8N0X2