POLDIP2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
POLDIP2 antibody was raised against a 13 amino acid synthetic peptide near the internal region (near C-Terminus) of POLDIP2 (near C-Terminus).
POLDIP2
Reactivity: Rat
WB, IF (cc), IF (p), IHC (fro), IHC (p), ICC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 37 kDa band observed in rat Vascular Smooth muscle Cells lysates (calculated MW of 15.2 kDa according to rat NP_001099286.1). The band dimished upon siRNA knock down. Recommended concentration: 1-3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
POLDIP2
(Polymerase (DNA-Directed), delta Interacting Protein 2 (POLDIP2))