NOX1 antibody (Internal Region)

Catalog No. ABIN2464425

Product Details anti-NOX1 Antibody

Target: NOX1 (NADPH Oxidase 1 (NOX1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This NOX1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: This antibody is expected to recognize both reported isoforms (NP_008983.2, NP_39249.1).

Purification: NOX1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: NOX1 antibody was raised against a 13 amino acid synthetic peptide near the internal region of NOX1.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 65 kDa band observed in human and mouse colon lysates (calculated MW of 64.9 kDa according to NP_008983.2). The 65 kDa was also seen in cultured transgenic mouse Aortic Smooth muscle cells (MASM), but not to be seen at endogenous levels in MASM. Recommended concentration: 1-3 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for NOX1

Target: NOX1 (NADPH Oxidase 1 (NOX1))

Alternative Name: NOX1 (NOX1 Products)

Synonyms: GP91-2 antibody, MOX1 antibody, NOH-1 antibody, NOH1 antibody, NOX1a antibody, NOX1alpha antibody, Nox-1 antibody, Nox1 antibody, NADPH oxidase 1 antibody, NOX1 antibody, Nox1 antibody, nox1 antibody

Gene ID: 27035

NCBI Accession: NP_008983, NP_39249

UniProt: Q9Y5S8

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Proton Transport