The immunizing peptide represents part of a potential extracellular domain.
Purification
Tnfrsf1b antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Tnfrsf1b antibody was raised against a 14 amino acid synthetic peptide near the internal region of Tnfrsf1b (mouse).
TNFRSF1B
Reactivity: Mouse
WB, FACS, IP, Func, IA
Host: Rat
Monoclonal
HM102
Biotin
Application Notes
ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 55 kDa band observed in mouse and rat spinal cord lysates (calculated MW of 50.3 kDa according to mouse NP_035740.2). Recommended concentration: 0.2-0.6 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
TNFRSF1B
(Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))