This antibody is expected to recognize both reported isoforms (NP_835471.1, NP_001186976.1).
Purification
Nmnat3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Nmnat3 antibody was raised against a 15 amino acid synthetic peptide near the internal region of Nmnat3 (mouse).
NMNAT3
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
HRP
Application Notes
ELISA: Antibody detection limit dilution 1:64000. Western Blot: Approximately 28 kDa band observed in mouse heart lysates (calculated MW of 27.7 kDa according to NP_653116.1). Recommended concentration: 0.5-2 μg/mL. an additional strong band of unknown identity was also consistently observed at 75 kDa . This band was successfully blocked by incubation with the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.