NFASC antibody (Internal Region)

Catalog No. ABIN2464498

Product Details anti-NFASC Antibody

Target: NFASC (Neurofascin (NFASC))

Binding Specificity: Internal Region

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This NFASC antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: This antibody is expected to recognize reported isoforms 1, 2, 3 and 4 (NP_001005388.2,NP_001153803.1, NP_001153804.1and NP_055905.2 resp) .

Purification: NFASC antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: NFASC antibody was raised against a amino acid synthetic peptide near the internal region of NFASC (aa 877-890).

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 170 kDa band observed in human lung and rat brain lysates (calculated MW of 138 kDa according to NP_001005388.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kirschbaum et al, J Biol Chem. 2009 Oct 16,284 (42) :28533-42. PMID: 19666467). Recommended concentration: 0.5-2 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for NFASC

Target: NFASC (Neurofascin (NFASC))

Alternative Name: NFASC (NFASC Products)

Synonyms: sc:d0205 antibody, NF antibody, NRCAML antibody, AA387016 antibody, D430023G06Rik antibody, mKIAA0756 antibody, neurofascin antibody, neurofascin homolog (chicken) a antibody, NFASC antibody, nfasca antibody, Nfasc antibody

Gene ID: 23114

NCBI Accession: NP_001005388, NP_001153803, NP_001153804, NP_055905

UniProt: O94856

Pathways: Cell-Cell Junction Organization