Serpina6 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Serpina6 antibody was raised against a 14 amino acid synthetic peptide near the internal region of Serpina6 (rat).
Verified
SERPINA6
Reactivity: Rat
WB, ELISA
Host: Goat
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 55 kDa band observed in rat liver lysates (calculated MW of 44.7 kDa according to NP_001009663.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Richard et al, Endocrinology. 2010 Feb,151 (2) :649-59, PMID: 20022933). Recommended concentration: 0.03-0.1 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
SERPINA6
(serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 6 (SERPINA6))