This antibody is expected to recognize both isoforms (NP_001090.2, NP_001127666.1).
Purification
ACPP antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
ACPP antibody was raised against a 13 amino acid synthetic peptide near the internal region of ACPP.
ELISA: Antibody detection limit dilution 1:64000 Western Blot: Approximately 50 kDa band observed in human prostate lysates (calculated MW of 44.6 kDa according to NP_001090.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Patel et al,Clin Chem. 1986 Oct,32 (10) :1832-5. PMID: 3530530). Recommended concentration: 0.3-1 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.