This antibody is expected to recognize isoforms 1 and 2 (NP_000296.2, NP_001018171.1).
Purification
PON2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
PON2 antibody was raised against an 11 amino acid synthetic peptide near the internal region of PON2.
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 40 kDa double band observed in lysates of HepG2 (calculated MW of 39.4 kDa according to NP_000296.2 and of 38.0 kDa according to NP_001018171.1). Recommended concentration: 0.2-0.6 μg/mL. an additional band of unknown identity was also consistently observed at 60 kDa . This band was successfully blocked by incubation with the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Capable of hydrolyzing lactones and a number of aromatic carboxylic acid esters. Has antioxidant activity. Is not associated with high density lipoprotein. Prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis.