SOX3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
SOX3 antibody was raised against a 15 amino acid synthetic peptide near the C-Terminus of SOX3.
SOX3
Reactivity: Human
WB, ELISA, IHC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:8000 Western Blot: Approximately 42 kDa band observed in human brain (cerebellum, cerebral cortex and frontal cortex) lysates (calculated MW of 45.2 kDa according to NP_005625.2). Recommended concentration: 0.5-1.5 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
SOX3
(SRY (Sex Determining Region Y)-Box 3 (SOX3))